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Restiction Enzyme Background

 

A restriction enzyme's purpose is to cut DNA at a specific site in order to isolate genes and transplant foreign ones within an organism. This unique tool allows us to improve upon genes and transplant genes across species; a modern example of this is the implementation of an insulin-producting trade into the E. Coli bacteria. Restriction enzymes are usually classified into three different types, which differ in both their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. What the restiction enzyme cuts is later put back together with the enzyme Ligase. 

 

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RESTRICTION ENZYMES

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THE GENE GUN

Many people have the question: how do you get a plant to take up a specific gene? One answer is the gene gun. A gene gun is a device used to inject cells with genetic information. These guns contain DNA-coated metallic bullets and goes into the walls and membranes of the cells. Once it gets within the cell the DNA that covers the bullets will act like a virus and start making copies of itself by conducting mitosis. This will then be cultivated in a petri dish until they get the desired product.   

 

 

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